Valentina R Dikova
University of Valencia, Spain
Title: Identifi cation of Potential Salivary Biomarkers for early OSCC
Biography
Biography: Valentina R Dikova
Abstract
Oral Squamous Cell Carcinoma (OSCC) is a common human malignancy with poor survival rates associated with the late diagnosis and frequent recurrences. It develops through a multistep process where the initial presence of a precursor cell subsequently evolving into a tumor outgrowth is well established. Furthermore, a complex of genetic and epigenetic metabolic changes has been correlated to the cancerous transformation of oral potentially malignant disorders (OPMD) such as Oral Leukoplakia (OL). Since inflammation has been linked to the pathology of OSCC, research to date indicates the possibility of using salivary pro and anti-inflammatory factors for screening of oral disorders. Therefore, a prospective study to determine novel molecular biomarkers is carried out addressing current clinical needs with diagnostic & prognostic means. Multiplex immunoassay enabled simultaneous detection and quantitation of a protein panel including IL-1α, IL-6, IL-8, IP-10, MCP-1, TNF-α, HCC-1, PF-4 and MIP-4 in saliva samples obtained from 86 volunteers (mean age 64.4±3.86 years) classified into Control, OL, Early and Advanced stage of OSCC groups. Analysis showed significantly elevated concentrations of IL-6, IL-8, IL-10, TNF-α, HCC-1, MIP-4 and PF-4 according to the oral cancer progression (Fig.1A,B). Besides, MCP-1 and PF-4 were found importantly higher in pre-cancerous condition collated to controls, while IL-8 and PF-4 demonstrated a trend towards a growth among patients diagnosed with OL and OSCC (Fig.2A, B). The results suggest that saliva can be used as a promising diagnostic fl uid where measureable parameters like cytokines and chemokines at differential levels can discriminate disease process and have a prognostic value.