Pritsana Raungrut
Prince of Songkla University, Thailand
Title: Expression of 14-3-3γ provtein induces the migration and invasion of lung cancer cells
Biography
Biography: Pritsana Raungrut
Abstract
14-3-3γ has been shown to be involved in the metastasis of lung cancer; however, its functional roles regarding an ability of tumor cell invasion are still unclear. In this study, the roles of 14-3-3γ in migration and invasion were investigated using NSCLC cells. The constructed 14-3-3γ expression vector was used to increase the expression level of 14-3-3γ. Expression levels of proteins were detected by western blotting. Cellular migration and invasion was analyzed using 2D/3D wound healing model and Trans well assay. The wound healing assay revealed that the closing rate of scratch wounds in invasion was significantly increased in 14-3-3γ-overexpressing cells compared with the non-transfected cells (33%, P=0.001 at 6 h and 30%, P=0.009 at 24 h) and the vector control cells (36%, P=0.001 at 6 h and 18%, P=0.015 at 24 h). Similarly, overexpression of 14-3-3γ resulted in a significant increase in migration by approximately 25% (P=0.048) and 32% (P=0.002) at 24 h compared with the non-transfected and the vector control cells, respectively. For Transwell assay, the result showed that the 14-3-3γ-overexpressing cells also led to a significant increase in the invasion and migration compared with the non-transfected Cells by approximately 79% (P=0.002) and 131% (P=0.001), respectively. Our results suggested that overexpression of 14-3-3γ promotes the migration and invasion of NSCLC and it may provide new insight for treatment of NSCLC.